Development of Efficient Callus Initiation of Malta (Citrus sinensis) Through Tissue Culture

Authors

  • Fazle Azim Department of Genetic Engineering & Biotechnology, Shahjalal University of Science & Technology
  • MM Rahman Department of Genetic Engineering & Biotechnology, Shahjalal University of Science & Technology
  • Shamsul H Prodhan Department of Genetic Engineering & Biotechnology, Shahjalal University of Science & Technology
  • Saif U Sikdar Department of Genetic Engineering & Biotechnology, Shahjalal University of Science & Technology
  • Nayem Zobayer Department of Genetic Engineering & Biotechnology, Shahjalal University of Science & Technology
  • M Ashrafuzzaman Department of Genetic Engineering & Biotechnology, Shahjalal University of Science & Technology

Keywords:

Malta, Citrus sinensis, In vitro, Seed shoot formation, Callus induction

Abstract

The effects of different hormonal concentration on shoot formation and callus induction were studied on BARI Malta-1 (Citrus sinensis). Seeds containing seed coat and without seed coat was treated by Murashige and Skoog (MS) media supplemented with 6-benzyl adenine (BA) and Kinetin (KIN). Removal of seed coat showed an early response for shoot formation. The highest (70%) shoot formation was obtained from seeds without seed coat treated with MS basal media + BA 1.0 mg/l while KIN showed no response for shoot formation in any supplemented concentration. However, in case of callus induction internodes and apical shoot tips were used as explants and 2, 4-dichlorophenoxy acetic acid (2, 4-D) was used as callus inducing hormone. MS basal media supplemented with 2, 4-D, 2.0 mg/l showed highest (68%) callus induction.

DOI: http://dx.doi.org/10.3329/ijarit.v1i1-2.13935

Int. J. Agril. Res. Innov. & Tech. 1 (1&2): 64-68, December, 2011

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Published

2013-02-23

How to Cite

Development of Efficient Callus Initiation of Malta (Citrus sinensis) Through Tissue Culture. (2013). International Journal of Agricultural Research, Innovation and Technology, 1(1-2), 64-68. https://doi.org/10.3329/ijarit.v1i1-2.13935

Issue

Vol. 1 No. 1-2 (2011)

Section

Articles

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  • Copyright on any research article published in the International Journal of Agricultural Research, Innovation and Technology is retained by the authors and grants any third party the right to use, reproduce, and disseminate the article.
  • Articles in the International Journal of Agricultural Research, Innovation and Technology are Open Access articles published under the Creative Commons CC BY License (https://creativecommons.org/licenses/by/4.0/)
  • This license permits use, distribution, and reproduction in any medium, provided the original work is properly cited.

How to Cite

Development of Efficient Callus Initiation of Malta (Citrus sinensis) Through Tissue Culture. (2013). International Journal of Agricultural Research, Innovation and Technology, 1(1-2), 64-68. https://doi.org/10.3329/ijarit.v1i1-2.13935